Semi-nested polymerase chain reaction-based detection of Babesia spp in small ruminants from Northwest of Iran

Semi-nested polymerase chain reaction-based detection of Babesia spp. in small ruminants from Northwest of Iran


چاپ صفحه
پژوهان
صفحه نخست سامانه
چکیده مقاله
چکیده مقاله
نویسندگان
نویسندگان
دانلود مقاله
دانلود مقاله
دانشگاه علوم پزشکی تبریز
دانشگاه علوم پزشکی تبریز

نویسندگان: احد بازمانی , عباس ایمانی باران

کلمات کلیدی: Babesia motasi, Babesia ovis, Iran, semi-nested polymerase chain reaction, small ruminants.

نشریه: 34549 , 3 , 11 , 2018

اطلاعات کلی مقاله
hide/show

نویسنده ثبت کننده مقاله احد بازمانی
مرحله جاری مقاله تایید نهایی
دانشکده/مرکز مربوطه بیماری های عفونی و گرمسیری
کد مقاله 62469
عنوان فارسی مقاله Semi-nested polymerase chain reaction-based detection of Babesia spp in small ruminants from Northwest of Iran
عنوان لاتین مقاله Semi-nested polymerase chain reaction-based detection of Babesia spp. in small ruminants from Northwest of Iran
ناشر 2
آیا مقاله از طرح تحقیقاتی و یا منتورشیپ استخراج شده است؟ خیر
عنوان نشریه (خارج از لیست فوق)
نوع مقاله Original Article
نحوه ایندکس شدن مقاله ایندکس شده سطح یک – ISI - Web of Science
آدرس لینک مقاله/ همایش در شبکه اینترنت http://www.veterinaryworld.org/Vol.11/March-2018/2.html

خلاصه مقاله
hide/show

Aim: The present study aimed to detect Babesia ovis and Babesia motasi in the blood samples of sheep and goats from Northwest of Iran by the semi-nested polymerase chain reaction (PCR) technique. Materials and Methods: A total of 166 whole blood samples (including 123 sheep and 43 goats) were collected. In the first stage, the PCR was performed to amplify a piece of 18S rRNA gene of Babesia and Theileria genera. Then, semi-nested PCR was carried out on all PCR products to differentiate B. ovis and B. motasi. Results: The PCR indicated that totally, 19 (11.44%) out of 166 samples were positive for Babesia or Theileria spp. The semi-nested PCR showed that 38 samples (22.89%) were positive only for B. ovis. No significant association was found between the infection rate of B. ovis and age, gender and species of animals. Conclusion: In the present study, there was no evidence for B. motasi infection in small ruminants from Northwest of Iran. Therefore, B. ovis was the main causative agent of ovine Babesiosis in this region.

نویسندگان
hide/show

نویسنده نفر چندم مقاله
احد بازمانیاول
عباس ایمانی بارانسوم

لینک دانلود مقاله
hide/show

نام فایل تاریخ درج فایل اندازه فایل دانلود
4_5823543042795111365.pdf1396/12/141040200دانلود