Semi-nested polymerase chain reaction-based detection of Babesia spp in small ruminants from Northwest of Iran
Semi-nested polymerase chain reaction-based detection of Babesia spp. in small ruminants from Northwest of Iran
نویسندگان: احد بازمانی , عباس ایمانی باران
کلمات کلیدی: Babesia motasi, Babesia ovis, Iran, semi-nested polymerase chain reaction, small ruminants.
نشریه: 34549 , 3 , 11 , 2018
| نویسنده ثبت کننده مقاله |
احد بازمانی |
| مرحله جاری مقاله |
تایید نهایی |
| دانشکده/مرکز مربوطه |
بیماری های عفونی و گرمسیری |
| کد مقاله |
62469 |
| عنوان فارسی مقاله |
Semi-nested polymerase chain reaction-based detection of Babesia spp in small ruminants from Northwest of Iran |
| عنوان لاتین مقاله |
Semi-nested polymerase chain reaction-based detection of Babesia spp. in small ruminants from Northwest of Iran |
| ناشر |
2 |
| آیا مقاله از طرح تحقیقاتی و یا منتورشیپ استخراج شده است؟ |
خیر |
| عنوان نشریه (خارج از لیست فوق) |
|
| نوع مقاله |
Original Article |
| نحوه ایندکس شدن مقاله |
ایندکس شده سطح یک – ISI - Web of Science |
| آدرس لینک مقاله/ همایش در شبکه اینترنت |
http://www.veterinaryworld.org/Vol.11/March-2018/2.html |
| Aim: The present study aimed to detect Babesia ovis and Babesia motasi in the blood samples of sheep and goats from
Northwest of Iran by the semi-nested polymerase chain reaction (PCR) technique.
Materials and Methods: A total of 166 whole blood samples (including 123 sheep and 43 goats) were collected. In the first
stage, the PCR was performed to amplify a piece of 18S rRNA gene of Babesia and Theileria genera. Then, semi-nested
PCR was carried out on all PCR products to differentiate B. ovis and B. motasi.
Results: The PCR indicated that totally, 19 (11.44%) out of 166 samples were positive for Babesia or Theileria spp. The
semi-nested PCR showed that 38 samples (22.89%) were positive only for B. ovis. No significant association was found
between the infection rate of B. ovis and age, gender and species of animals.
Conclusion: In the present study, there was no evidence for B. motasi infection in small ruminants from Northwest of Iran.
Therefore, B. ovis was the main causative agent of ovine Babesiosis in this region. |
| نام فایل |
تاریخ درج فایل |
اندازه فایل |
دانلود |
| 4_5823543042795111365.pdf | 1396/12/14 | 1040200 | دانلود |