چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده ثبت کننده مقاله | بهزاد برادران |
| مرحله جاری مقاله | تایید نهایی |
| دانشکده/مرکز مربوطه | مرکز تحقیقات ایمونولوژی |
| کد مقاله | 62351 |
| عنوان فارسی مقاله | An analysis of suppressing migratory effect on human urinary bladder cancer cell line by silencing of snail-1 |
| عنوان لاتین مقاله | An analysis of suppressing migratory effect on human urinary bladder cancer cell line by silencing of snail-1 |
| ناشر | 8 |
| آیا مقاله از طرح تحقیقاتی و یا منتورشیپ استخراج شده است؟ | بلی |
| عنوان نشریه (خارج از لیست فوق) | Biomedicine & Pharmacotherapy |
| نوع مقاله | Original Article |
| نحوه ایندکس شدن مقاله | ایندکس شده سطح یک – ISI - Web of Science |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | https://www.sciencedirect.com/science/article/pii/S075333221733514X |
| Background: Snail-1 actively participates in tumor progression, invasion, and migration. Targeting snail-1 expression can suppress the EMT process in cancer. The aim of this study was to investigate the effect of snail1 silencing on urinary bladder cancer. Methods: Quantitative RT-PCR was used to detect snail-1 and other related metastatic genes expression following siRNA knockdown in urinary bladder cancer EJ-138 cells. The protein level of snail1 was assessed by Western blot. MTT and TUNEL assays were assessed to understand if snail-1 had survival effects on EJ-138 cells. Scratch wound healing assay measured cell motility effects after snail1 suppression. Results: The significant silencing of snail-1 reached 60 pmol siRNA in a 48-h post-transfection. The result of scratch assay showed that snail-1 silencing significantly decreased Vimentin, MMPs, and CXCR4 expression; however, expression of E-cadherin was induced. The cell death assay indicated that snail-1 played the crucial role in bladder cancer survival rate. Conclusion: These results propose that snail-1 plays a major role in the progression and migration of urinary bladder cancer, and can be a potential therapeutic target for target therapy of invasive urinary bladder cancer. |
| نویسنده | نفر چندم مقاله |
|---|---|
| بهزاد منصوری | دوم |
| علی محمدی | سوم |
| محمد حسین موسوی شناس | پنجم |
| ندا شجری | ششم |
| جعفر مجیدی ذوالبین | هفتم |
| بهزاد برادران | هشتم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| 192-An analysis of suppressing migratory effect on human urinary bladder.pdf | 1396/11/26 | 750713 | دانلود |
