The effect of ketorolac and triamcinolone acetonide on adipogenic and hepatogenic differentiation through miRNAs 16/15/195: Possible clinical application in regen..

zarghami, nosratollah; Alizadeh, Effat; Fekri Aval, Sedigheh

doi:https://doi.org/10.1016/j.biopha.2017.10.126

اطلاعات کلی مقاله

نویسنده ثبت کننده مقاله	نصرت اله ضرغامی
مرحله جاری مقاله	تایید نهایی
دانشکده/مرکز مربوطه	مرکز سلولهای بنیادی
کد مقاله	61792
عنوان فارسی مقاله	The effect of ketorolac and triamcinolone acetonide on adipogenic and hepatogenic differentiation through miRNAs 16/15/195: Possible clinical application in regen..
عنوان لاتین مقاله	The effect of ketorolac and triamcinolone acetonide on adipogenic and hepatogenic differentiation through miRNAs 16/15/195: Possible clinical application in regen..
ناشر	4
آیا مقاله از طرح تحقیقاتی و یا منتورشیپ استخراج شده است؟	خیر
عنوان نشریه (خارج از لیست فوق)	Biomedicine & Pharmacotherapy
نوع مقاله	Original Article
نحوه ایندکس شدن مقاله	ایندکس شده سطح یک – ISI - Web of Science
آدرس لینک مقاله/ همایش در شبکه اینترنت	http://www.sciencedirect.com/science/article/pii/S0753332217346073

خلاصه مقاله

Objective The role of miR-16/15b/195 and complementary compounds in the commitment of mesenchymal stem cells (MSCs) to different lineages is unknown. The aim of this work was to investigate the effect of ketorolac and triamcinolone acetonide on adipogenic and hepatogenic processes, respectively. Also, miR-16/15 and miR-195 expression levels were evaluated under different induction conditions. Methods MSCs were isolated, expanded and directed using adipogenesis medium or medium supplemented with ketorolac, and also subjected to hepatogenic differentiation using a cocktail of hepatocyte growth factor (HGF) and Oncostatin M (OSM) either with or without triamcinolone acetonide. Periodic acid-Schiff (PAS) staining, Oil red O staining, albumin and adiponectin protein secretion were evaluated. MiR-16 family expression level was assessed using qRT-PCR in four induced groups as compared to non-induced cells. Results The expression levels of miR-16 and miR-15 increased significantly from adipose derived stem cells to adipocytes (p < 0.01). Positive stimulatory effects of ketorolac and triamcinolone on adipogenic and hepatogenic induction, respectively, were observed. Ketorolac stimulated upregulation of miR-16/15b in the adipogenic induced stem cells. Interestingly, triamcinolone caused upregulation of miR-15b and miR-195 in hepatic commitment. Conclusions Two highly effective stimulators were identified. Ketorolac is similar to indomethacin and affects adipogenic differentiation. Triamcinolone is involved in hepatogenic commitment of stem cells like differentiation process of macrophages, adipocytes and osteocytes. The alteration of miR-16 family members’ expression indicates that this family may play a possible role in directing stem cell fate.

نویسندگان

نویسنده	نفر چندم مقاله
نصرت اله ضرغامی	دوم
عفت علیزاده	سوم
صدیقه فکری اول	اول

لینک دانلود مقاله

نام فایل	تاریخ درج فایل	اندازه فایل	دانلود
The effect of ketorolac and triamcinolone acetonide on adipogenic and hepatogenic differentiation through miRNAs.pdf	1396/08/22	1619436	دانلود

The effect of ketorolac and triamcinolone acetonide on adipogenic and hepatogenic differentiation through miRNAs 16/15/195: Possible clinical application in regen..