چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده | نفر چندم مقاله |
|---|---|
| مریم اکبرزاده | اول |
| عنوان | متن |
|---|---|
| کلمات کلیدی | Cancer stem cells, Ovarian cancer, Melatonin, Invasion, Metastasis |
| خلاصه مقاله | Introduction: Ovarian cancer is the fifth most common cancer in women. Metastasis and recurrence are the main reason for high mortality rate in these patients. Cancer stem cells are thought to contribute to tumor recurrence and metastasis in many cancers. In this study we have investigated the role of anti-invasive effects of melatonin as a natural hormone in ovarian cancer stem cells (CSCs). Methods: Isolation of CSCs was performed by MACS. Self-renewal ability was evaluated by spheroid formation and SOX2 protein expression. MTT assay and flow cytometry of ki67 expression were applied to determine CSCs proliferation rate under melatonin treatment. Invasion was assessed by matrix metalloproteinase 2 and 9 and EMT-related gene expressions by real time RT-PCR. The activity of MMP2 and MMP9 also were assessed by zymography. Cell migration and related signaling pathways were analyzed by Transwell migration assay. Results: Isolated CSCs showed the ability of spheroid formation along with SOX2 overexpression. Melatonin (3.4 mM) inhibited proliferation of CSCs by 23% which was confirmed by a marked decrease in protein expression of Ki67 (p<0.05). Applying luzindole abolished anti-proliferative effect of melatonin by 12.33% and 17.59% in SKOV3 and CSCs respectively. Melatonin also decreased EMT related gene expressions increase in E-cadherin gene (p<0.05). Incubation of CSCs with melatonin showed a marked decrease in MMP9 expression and activity without any significant change in MMP-2 activity (p<0.05). Melatonin also inhibited CSCs migration in a partially receptor dependent manner. Melatonin induced suppression in migration of CSCs with no effect on PI3K and MAPK signaling pathways. Conclusion: Our results suggest that melatonin as a natural compound, can be considered as an important adjutant to control invasion, metastasis and proliferation of CSCs. |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Ovarian cancer stem cell.docx | 1396/08/22 | 13471 | دانلود |
| Maryam Akbarzadeh.pdf | 1396/08/22 | 233077 | دانلود |
