چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده ثبت کننده مقاله | نصرت اله ضرغامی |
| مرحله جاری مقاله | تایید نهایی |
| دانشکده/مرکز مربوطه | دانشکده علوم نوین پزشکی |
| کد مقاله | 61772 |
| عنوان فارسی مقاله | The inhibitory effects of nano-encapsulated metformin on growth and hTERT expression in breast cancer cells |
| عنوان لاتین مقاله | The inhibitory effects of nano-encapsulated metformin on growth and hTERT expression in breast cancer cells |
| ناشر | 7 |
| آیا مقاله از طرح تحقیقاتی و یا منتورشیپ استخراج شده است؟ | خیر |
| عنوان نشریه (خارج از لیست فوق) | |
| نوع مقاله | Original Article |
| نحوه ایندکس شدن مقاله | ایندکس شده سطح یک – ISI - Web of Science |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | http://www.sciencedirect.com/science/article/pii/S1773224717304495 |
| Metformin has recently received attention for its potential effects with respect to cancer prevention and treatment. Nonetheless, the low bioavailability and short half-life of metformin hamper its application as an anti-cancer drug. The present study aims to evaluate the efficiency of PLGA-PEG as a nanocarrier for metformin to enhance anticancer effects. For this purpose, metformin-loaded PLGA-PEG NPs were synthetized and characterized using DLS, FE-SEM and FTIR. Then, the inhibitory effects of free and nano-encapsulated forms of metformin on growth and expression levels of hTERT in two breast cancer cell lines, T47D and MDA-MB-231, were evaluated using MTT and qPCR assays, respectively. Assessment of drug toxicity revealed that metformin-loaded NPs had more cytotoxic effects than free form in both cell lines at a dose- and time-dependent manner. The IC50s of free and nanoformulated metformin for MDA-MB-231 cells were lower than T47D cells, indicating the higher sensitivity of the triple-negative phenotype. Also, it was found that nanoformulated metformin than free metformin could further decline the expression levels of hTERT in both cell lines, especially MDA-MB-231 (p < 0.05). It is speculated that nano-encapsulation of metformin into polymeric NPs may be a promising and convenient approach to improve its efficiency in breast cancer therapy. |
| نویسنده | نفر چندم مقاله |
|---|---|
| نصرت اله ضرغامی | هفتم |
| یونس پیله ور سلطان احمدی | دوم |
| وحید شفیعی ایران نژاد | پنجم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| The inhibitory effects of nano-encapsulated metformin on growth andhTERT expression in breast cancer cells.pdf | 1397/05/14 | 1724377 | دانلود |
