چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده ثبت کننده مقاله | فریبا حشمتی افشار |
| مرحله جاری مقاله | تایید نهایی |
| دانشکده/مرکز مربوطه | مرکز تحقیقات کاربردی دارویی |
| کد مقاله | 61616 |
| عنوان فارسی مقاله | In-vitro Bioactivity and Phytochemical Screening of Extracts from Rhizomes of Eremostachys azerbaijanica rech. f. Growing in Iran |
| عنوان لاتین مقاله | In-vitro Bioactivity and Phytochemical Screening of Extracts from Rhizomes of Eremostachys azerbaijanica rech. f. Growing in Iran |
| ناشر | 6 |
| آیا مقاله از طرح تحقیقاتی و یا منتورشیپ استخراج شده است؟ | بلی |
| عنوان نشریه (خارج از لیست فوق) | |
| نوع مقاله | Original Article |
| نحوه ایندکس شدن مقاله | ایندکس شده سطح یک – ISI - Web of Science |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | http://ijpr.sbmu.ac.ir/article_1960_442e66838315d96cc4e20951652282f8.pdf |
| The current study evaluated the general toxicity, antioxidant, antimicrobial, and cytotoxic activity of extracts obtained from the rhizomes of Eremostachys azerbaijanica (Labiatae) as well as analyzed the potent extracts using GC-MS. Extracts of E. azerbaijanica in n-hexane, dichloromethane (DCM) and methanol (MeOH) were prepared using a Soxhlet apparatus. The antioxidant activity of the extracts was evaluated for free radical scavenging activity by DPPH assay. The antimicrobial activity of samples was determined by disc diffusion and brine shrimp lethality assay (BSLA) was used to assess general toxicity. The cytotoxicity of each extract was determined by MTT assay against human colorectal adenocarcinoma (HT29), human lung carcinoma (A549) and a normal cell line (human umbilical vein endothelial cells, HUVEC). The MeOH extract showed significant antioxidant activity and the n-hexane and DCM extracts showed promising activity against gram-positive species when compared with amikacin as a standard. Moreover, the n-hexane extract displayed the most potent activity in general toxicity assay. The results showed that all three extracts have cytotoxic effects against the A549 cell line. In the case of HT29 cell lines, only the DCM extract exhibited cytotoxicity. Interestingly, none of the extracts showed significant cytotoxic activity against the HUVEC cell line. The bioassay-guided identification of constituents showed the presence of fatty acids and steroids as the compounds responsible for bioactivity in the non-polar extracts. |
| نویسنده | نفر چندم مقاله |
|---|---|
| سولماز اثناعشری | اول |
| عباس دل آذر | دوم |
| پرینا اصغریان | سوم |
| فرزانه لطفی پور بناب | چهارم |
| صدیقه بامدادمقدم | پنجم |
| فریبا حشمتی افشار | ششم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| 2017-IJPR.pdf | 1396/08/02 | 351738 | دانلود |
