Evaluation of Antibacterial Efficacy of Photodynamic Therapy vs. 2.5% NaOCl against E. faecalis-infected Root Canals Using Real-time PCR Technique
Evaluation of Antibacterial Efficacy of Photodynamic Therapy vs. 2.5% NaOCl against E. faecalis-infected Root Canals Using Real-time PCR Technique
نویسندگان: مریم جانانی , فرناز جعفری , محمد سمیعی , فرزانه لطفی پور بناب , نگین قاسمی , آیلار نخلبند , طناز سالاری
کلمات کلیدی: Photoactivated laser, Enterococcus faecalis, antibacterial agents, sodium hypochlorite
نشریه: 19695 , 4 , 9 , 2017
| نویسنده ثبت کننده مقاله |
مریم جانانی |
| مرحله جاری مقاله |
تایید نهایی |
| دانشکده/مرکز مربوطه |
دانشکده دندانپزشکی |
| کد مقاله |
61588 |
| عنوان فارسی مقاله |
Evaluation of Antibacterial Efficacy of Photodynamic Therapy vs. 2.5% NaOCl against E. faecalis-infected Root Canals Using Real-time PCR Technique |
| عنوان لاتین مقاله |
Evaluation of Antibacterial Efficacy of Photodynamic Therapy vs. 2.5% NaOCl against E. faecalis-infected Root Canals Using Real-time PCR Technique |
| ناشر |
7 |
| آیا مقاله از طرح تحقیقاتی و یا منتورشیپ استخراج شده است؟ |
بلی |
| عنوان نشریه (خارج از لیست فوق) |
|
| نوع مقاله |
Original Article |
| نحوه ایندکس شدن مقاله |
ایندکس شده سطح دو – PubMed |
| آدرس لینک مقاله/ همایش در شبکه اینترنت |
https://www.ncbi.nlm.nih.gov/pubmed/?term=Evaluation+of+Antibacterial+Efficacy+of+Photodynamic+Therapy+vs.+2.5%25+NaOCl+against+ |
| Background: Bacteria like E. faecalis can produce intra- and extra-radicular biofilms. Theoretically, the adjustable
penetration ability of lasers enables better access to root canal system. Therefore the aim of the present study was
to compare the ability of photoactivated laser and 2.5% NaOCl irrigation solution to eliminate E. faecalis from the
root canals by real-time PCR technique.
Material and Methods: Sixty extracted human upper central incisors were selected and sterilized in an autoclave.
The root canals were infected with E. faecalis (PTCC 1237, Persian Type Culture Collection, Iran) and then incubated
for 24 hours. The samples were randomly divided into 3 groups. No intervention was made in the control
group (group 1). In group 2, laser therapy was performed with a power of 100 mW by diode laser for 120 seconds.
In group 3, the canals were irrigated with 5 mL of 2.5% NaOCl; then all the samples were sonicated in 15 mL of
normal saline in test tubes in order to isolate the bacteria. DNA extraction was performed followed by real-time
PCR technique for all the samples.
Results: Inhibition of bacterial growth in all the experimental samples was significantly more than that in the
control group. There was a significant difference between photodynamic therapy and 2.5% NaOCl. The effect of
NaOCl in all the samples was better than photodynamic therapy. The results of the mean CT (cyclic threshold) were
40, 30.2 and 15.35 for 2.5% NaOCl, photodynamic therapy and control group, respectively.
Conclusions: Based on the results of this experimental study, 2.5% NaOCl eliminated E. faecalis from infected root
canals more effectively compared to photodynamic therapy. |
| نام فایل |
تاریخ درج فایل |
اندازه فایل |
دانلود |
| 18.pdf | 1396/07/26 | 424252 | دانلود |