چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده ثبت کننده مقاله | رضا قوطاسلو |
| مرحله جاری مقاله | تایید نهایی |
| دانشکده/مرکز مربوطه | بیماری های عفونی و گرمسیری |
| کد مقاله | 61556 |
| عنوان فارسی مقاله | In vitro synergy of antibiotic combinations against planktonic and biofilm Pseudomonas aeruginosa |
| عنوان لاتین مقاله | In vitro synergy of antibiotic combinations against planktonic and biofilm Pseudomonas aeruginosa |
| ناشر | 5 |
| آیا مقاله از طرح تحقیقاتی و یا منتورشیپ استخراج شده است؟ | بلی |
| عنوان نشریه (خارج از لیست فوق) | GMS Hygiene and Infection Control |
| نوع مقاله | Original Article |
| نحوه ایندکس شدن مقاله | ایندکس شده سطح یک – ISI - Web of Science |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | http://www.egms.de/en/journals/dgkh/ |
| Aim: The combination of different antimicrobial agents and subsequent synergetic effects may be beneficial in treatment of P. aeruginosa infections. The aim of the present study was to determine antibiotic susceptibility patterns of clinical isolates of P. aeruginosa and the effect of different antibiotic combinations against the multidrug-resistant, biofilm-producing bacterium P. aeruginosa. Methods: Thirty-six P. aeruginosa clinical isolates were evaluated. The disk diffusion method was performed to determine antibiotic susceptibility patterns according to the Clinical and Laboratory Standards Institute (CLSI) guidelines. The minimum inhibitory concentration of antimicrobial agents for the test organisms was determined by the broth microdilution method. To determine synergetic effects of the combinations of agents, the checkerboard assay and the fractional inhibitory concentration were used. The biofilm inhibitory concentration was determined to detect any inhibitory effect of antibiotics against the biofilm. Results: High levels of resistance were detected against most antibiotics, except colistin and polymyxin. According to the disk diffusion method, 58.3% of isolates were MDR. A synergetic effect between amikacin/ceftazidime, tobramycin/colistin and ceftazidime/colistin were found in 55.6%, 58.3% and 52.8% of isolates, respectively. A significant synergetic effect against biofilm-producing isolates was observed for the combination of tobramycin (0.5-1 µg/ml) and clarithromycin (256-512 µg/ml). Conclusion: Combinations of antibiotics have a different activity on the biofilm and planktonic forms of P. aeruginosa. Consequently, separate detection of antibacterial and antibiofilm effects of the antibiotic combinations may be useful in guiding the antibiotic therapy. |
| نویسنده | نفر چندم مقاله |
|---|---|
| محمد یوسف معمار | دوم |
| رضا قوطاسلو | پنجم |
| مینا یکانی | چهارم |
| فاطمه یگانه سفیدان | سوم |
| حسین قربانی | اول |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Ghorbanidgkh000302_print.pdf | 1396/07/20 | 1580249 | دانلود |
| questionnaire (2).doc | 1396/07/22 | 209920 | دانلود |
| Poropsal Payannameh.doc | 1396/07/22 | 186368 | دانلود |
| Doc1.docx | 1396/07/22 | 112583 | دانلود |
