چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده ثبت کننده مقاله | محمدرضا علی وند |
| مرحله جاری مقاله | تایید نهایی |
| دانشکده/مرکز مربوطه | دانشکده پزشکی |
| کد مقاله | 61397 |
| عنوان فارسی مقاله | Novel Epigenetic Controlling of Hypoxia Pathway Related toOverexpression and Promoter Hypomethylation of TET1 andTET2 in RPE Cell |
| عنوان لاتین مقاله | Novel Epigenetic Controlling of Hypoxia Pathway Related toOverexpression and Promoter Hypomethylation of TET1 andTET2 in RPE Cells |
| ناشر | 5 |
| آیا مقاله از طرح تحقیقاتی و یا منتورشیپ استخراج شده است؟ | خیر |
| عنوان نشریه (خارج از لیست فوق) | |
| نوع مقاله | Original Article |
| نحوه ایندکس شدن مقاله | ایندکس شده سطح یک – ISI - Web of Science |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | http://onlinelibrary.wiley.com/wol1/doi/10.1002/jcb.25965/abstract |
| CpG methylation of DNA takes part in a specific epigenetic memory that plays crucial roles in the differentiation and abnormality of the cells.The methylation pattern aberration of genomes is affected in three ways, namely DNA methyltransferase (DNMT), ten-eleven translocation(TET), and methyl-binding domain (MBD) proteins. Of these, TET enzymes have recently been demonstrated to be master modifier enzymes inthe DNA methylation process. Additionally, recent studies emphasize that not only epigenetic phenomena play a role in controlling hypoxiapathway, but the hypoxia condition also triggers hypomethylation of genomes that may help with the expression of hypoxia pathway genes. Inthis study, we suggested that TET1 and TET2 could play a role in the demethylation of genomes under chemical hypoxia conditions. Herein, theevaluating methylation status and mRNA expression of mentioned genes were utilized through real-time PCR and methylation-specific PCR(MSP), respectively. Our results showed that TET1 and TET2 genes were overexpressed (P < 0.05) under chemical hypoxia conditions in RetinalPigment Epithelial (RPE) cells, whereas the promoter methylation status of them were hypomethylated in the same condition. Therefore,chemical hypoxia not only causes overexpression of TET1 and TET2 but also could gradually do promoter demethylation of same genes. This isthe first study to show the relationship between epigenetics and the expression of mentioned genes related to hypoxia pathways. Furthermore,it seems that these associations in RPE cells are subjected to chemical hypoxia as a mechanism that could play a crucial role in methylationpattern changes of hypoxia-related diseases such as cancer and ischemia. |
| نویسنده | نفر چندم مقاله |
|---|---|
| محمدرضا علی وند | اول |
| سعید سلالی | چهارم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Alivand_et_al-2017-Journal_of_Cellular_Biochemistry(orignal).pdf | 1396/06/26 | 1543907 | دانلود |
