چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده ثبت کننده مقاله | محمد رضا توحیدکیا |
| مرحله جاری مقاله | تایید نهایی |
| دانشکده/مرکز مربوطه | مرکز تحقیقات ریز فناوری دارویی |
| کد مقاله | 61317 |
| عنوان فارسی مقاله | مطالعه کارایی و سمیت انتقال ژن بواسطه لیپید های کاتیونی به داخل رده سلولی سرطان معدهAGS |
| عنوان لاتین مقاله | Efficiency and cytotoxicity analysis of cationic lipids-mediated gene transfection into AGS gastric cancer cells. |
| ناشر | 2 |
| آیا مقاله از طرح تحقیقاتی و یا منتورشیپ استخراج شده است؟ | بلی |
| عنوان نشریه (خارج از لیست فوق) | ARTIFICIAL CELLS, NANOMEDICINE, AND BIOTECHNOLOGY |
| نوع مقاله | Original Article |
| نحوه ایندکس شدن مقاله | ایندکس شده سطح یک – ISI - Web of Science |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | http://www.tandfonline.com/doi/full/10.1080/21691401.2017.1355311 |
| In this effort, we provided comparative study on optimization of transfection conditions for AGS human gastric cancer cell line using two ommercial non-liposomal cationic lipids. Using reporter vector pEGFP-N1, transfection efficiency of AttracteneTM and X-tremeGENE HPTM ransfection reagents in terms of cell densities and DNA/reagent ratios was determined in AGS cells by flow cytometry and fluorescence icroscopy. In addition, influence of transfection reagents on direct cytotoxicity and cell viability was respectively, measured using lactate dehydrogenase LDH) leakage and MTT assays. Provided that the transfection rate of 29% and the mean fluorescence intensity of 437.5, the DNA/reagent ratio of .4/1.5 was selected as the optimal condition using AttracteneTM, whereas the optimum condition using X-tremeGENE HPTM was obtained by the ratio of 1/2 with a higher transfection rate of 36.9% and an MFI of 833. Very low direct cytotoxicity (<5% and 6–9% using AttracteneTM and X-remeGENE HPTM, respectively) and high cell viability (74.5–95.5% versus 68–75%) showed the biodegradable attribute for both transfection reagents. Altogether, X-tremeGENE HPTM exhibited superiority over AttracteneTM as a transfection reagent for AGS cells. In the present research, we have established the optimized protocols for efficient transfection of AGS cells with potential applications in gene function and expression studies as well as gene therapy. |
| نویسنده | نفر چندم مقاله |
|---|---|
| محمد رضا توحیدکیا | دوم |
| یداله امیدی | چهارم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| 10.1080@21691401.2017.1355311.pdf | 1396/10/24 | 1347085 | دانلود |
