| Themesenchymal stemcells (MSCs) are promising candidates for cell therapy due to the self-renewal, multi-potency,
ethically approved state and suitability for autologous transplantation.However, key issue for isolation and
manipulation ofMSCs is adhesion in ex-vivo culture systems. Biomaterials engineered formimicking natural extracellularmatrix
(ECM) conditionswhich support stemcell adhesion, proliferation and differentiation represent
a main area of research in tissue engineering. Some of them successfully enhanced cells adhesion and proliferation
because of their biocompatibility, biomimetic texture, and chemistry. However, it is still in its infancy, therefore
intensification and optimization of in vitro, in vivo, and preclinical studies is needed to clarify efficacies as
well as applicability of those bioengineered constructs. The aim of this review is to discuss mechanisms related
to the in-vitro adhesion of MSCs, surfaces biochemical, biophysical, and other factors (of cell's natural and artificial
micro-environment) which could affect it and a review of previous research attempting for its bio-chemooptimization |