چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده ثبت کننده مقاله | رویا صالحی قره ورن |
| مرحله جاری مقاله | تایید نهایی |
| دانشکده/مرکز مربوطه | دانشکده علوم نوین پزشکی |
| کد مقاله | 61128 |
| عنوان فارسی مقاله | Chrysin loaded nanostructured lipid carriers (NLCs) triggers apoptosis in MCF-7 cancer cells by inhibiting the Nrf2 pathway |
| عنوان لاتین مقاله | Chrysin loaded nanostructured lipid carriers (NLCs) triggers apoptosis in MCF-7 cancer cells by inhibiting the Nrf2 pathway |
| ناشر | 9 |
| آیا مقاله از طرح تحقیقاتی و یا منتورشیپ استخراج شده است؟ | خیر |
| عنوان نشریه (خارج از لیست فوق) | |
| نوع مقاله | Original Article |
| نحوه ایندکس شدن مقاله | ایندکس شده سطح یک – ISI - Web of Science |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | https://www.scopus.com/record/display.uri?eid=2-s2.0-85019871577&origin=resultslist&sort=plf-f&src=s&st1=salehi&st2=roya&nlo=1&n |
| We investigated the role of chrysin as an effective adjuvant along with nanostructured lipid carriers (NLCs) to increase the cytotoxicity of doxorubicin (Dox) in MCF-7 breast cancer cells. The prepared formulation was characterized from point of view scanning electron microscope (SEM), size & zeta potential. Cellular uptake and cytotoxicity of nanoparticles were examined by fluorescent microscopy and MTT assay, respectively. Flow cytometry and real-time PCR were used to understand the molecular mechanism of Nrf2 and related downregulating genes. The average size of the nanoparticles was 105 ± 2 nm, which was confirmed by SEM. Our results demonstrated that incubation of the cells with chrysin-loaded NLCs enhanced the percentage of apoptosis from 21.11 ± 5.72% to 27 ± 3.13% (p < 0.05). Furthermore, the population of cancer cells in the sub-G1 phase increased up to 12 ± 2.1% compared to untreated cells (p < 0.05). mRNA expression levels of Nrf2, NQO1, HO1, and MRP1 exhibited a significant decrease compared to the control group (p < 0.05). Our findings recommend that chrysin delivery along with NLCs would enhance the efficacy of Dox by exerting inhibitory effects against drug efflux pumps and drug detoxification enzymes. |
| نویسنده | نفر چندم مقاله |
|---|---|
| مهدی سبزیچی رادی | اول |
| جمال محمدیان | دوم |
| رویا بزاز دهخوارقانی | سوم |
| سید محمد باقر پیروز پناه | چهارم |
| مقصود شاکر | پنجم |
| حامد همیشه کار | ششم |
| سید هادی چاوشی | هفتم |
| رویا صالحی قره ورن | هشتم |
| ناصر صمدی | نهم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| 49-sabzichi-Process Biochemistry-2017.pdf | 1396/05/14 | 1244326 | دانلود |
