The herbal medicine Melissa officinalis extract effects on gene expression of p53, Bcl-2, Her2, VEGF-A and hTERT in human lung, breast and prostate cancer cell lines

Jahanban-Esfahlan, Rana; seidi, khaled; Shafiei-Irannejad, Vahid; Karimian, Ansar

doi:10.1016/j.gene.2017.02.034

اطلاعات کلی مقاله

نویسنده ثبت کننده مقاله	رعنا جهانبان اسفهلان
مرحله جاری مقاله	تایید نهایی
دانشکده/مرکز مربوطه	دانشکده علوم نوین پزشکی
کد مقاله	61116
عنوان فارسی مقاله	The herbal medicine Melissa officinalis extract effects on gene expression of p53, Bcl-2, Her2, VEGF-A and hTERT in human lung, breast and prostate cancer cell lines.
عنوان لاتین مقاله	The herbal medicine Melissa officinalis extract effects on gene expression of p53, Bcl-2, Her2, VEGF-A and hTERT in human lung, breast and prostate cancer cell lines
ناشر	7
آیا مقاله از طرح تحقیقاتی و یا منتورشیپ استخراج شده است؟	خیر
عنوان نشریه (خارج از لیست فوق)
نوع مقاله	Original Article
نحوه ایندکس شدن مقاله	ایندکس شده سطح یک – ISI - Web of Science
آدرس لینک مقاله/ همایش در شبکه اینترنت	https://www.ncbi.nlm.nih.gov/pubmed/28259690

خلاصه مقاله

Introduction: Earlier, we verified that Melissa officinalis extract (MOE) elicits potent antiproliferative effects on different human cancer cells. To gain insights into the molecular mechanisms accounting for the cytotoxic effects of MOE, we assessed the expression patterns of several prominent molecules with therapeutic potential in cancer by Quantitative PCR (Q-PCR). Methods: A549, MCF-7 and PC3 cancer cells were grown in complete RPMI 1640 and seeded in 24 well micro plates. After incubation for 72 h, 100µg/ml of MOE was added and the cells were further incubated for 72h. Afterwards, the cells were subjected to RNA extraction for the means of Q-PCR. Results: Our results indicated that in PC3 cancer cells, MOE resulted in a significant downregulation of VEGF-A (0.0004 fold), Bcl-2 (0.001 fold), Her2 (0.02 fold), and hTERT (0.023 fold) compared to the untreated control. In addition, VEGF-A and hTERT mRNA were significantly downregulated in MCF-7 and A549 cancer cells, as well. Notably, high antiangiogenic activity was closely associated with a high anti-telomerase activity of MOE in studying cancer cells. The decrease in VEGF-A expression was significantly superior than that of hTERT downregulation, as PC3 cancer cells with the highest hTERT down regulation (0.023)presented the highest anti VEGF activity (0.0004 fold), whereas MCF-7 cells with the lowest hTERT inhibition (0.213) showed the lowest VEGF inhibition(0.0435) among the three studied cancer cells. We noticed that the modulation of VEGF-A and hTERT gene expression can be considered as a common target, accounting for the therapeutic potential of MOE on human breast, lung and prostate cancer cells. Conclusion: Altogether, it is suggested that the potent antiproliferative activity of the hydroalcoholic extract of Melissa officinalis is somehow explainable by its high potency to inhibit expression of the prominent oncogenes Bcl2, Her2,VEGF-A and hTERT in prostate cancer. In tumors with functional p53, including MCF-7 and A549 cancer cells, the role of p53, Bcl2 and Her2 is less significant. It appears that MOE exerts its antiproliferative effects in these cancer cells partly via concurrent downregulation of VEGF-A and hTERT. Additional studies are needed to clarify the role of other active molecules in cancer cells harboring functional p53.

نویسندگان

نویسنده	نفر چندم مقاله
رعنا جهانبان اسفهلان	اول
خالد صیدی	دوم
وحید شفیعی ایران نژاد	چهارم
انصار کریمیان	ششم

لینک دانلود مقاله

نام فایل	تاریخ درج فایل	اندازه فایل	دانلود
jahanbanesfahlan2017_3.pdf	1396/05/12	677107	دانلود

The herbal medicine Melissa officinalis extract effects on gene expression of p53, Bcl-2, Her2, VEGF-A and hTERT in human lung, breast and prostate cancer cell lines.