چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده ثبت کننده مقاله | جعفر مجیدی ذوالبین |
| مرحله جاری مقاله | تایید نهایی |
| دانشکده/مرکز مربوطه | مرکز تحقیقات ایمونولوژی |
| کد مقاله | 61097 |
| عنوان فارسی مقاله | Cloning and molecular characterization of the cDNAs encoding the variable regions of an anti-CD20 monoclonal antibody. |
| عنوان لاتین مقاله | Cloning and molecular characterization of the cDNAs encoding the variable regions of an anti-CD20 monoclonal antibody. |
| ناشر | 5 |
| آیا مقاله از طرح تحقیقاتی و یا منتورشیپ استخراج شده است؟ | بلی |
| عنوان نشریه (خارج از لیست فوق) | |
| نوع مقاله | Original Article |
| نحوه ایندکس شدن مقاله | ایندکس شده سطح دو – Medline |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | http://content.iospress.com/articles/human-antibodies/hab314 |
| Abstract. BACKGROUND: CD20-based targeting of B-cells in hematologic malignancies and autoimmune disorders is associated with outstanding clinical outcomes. Isolation and characterization of VH and VL cDNAs encoding the variable regions of the heavy and light chains of monoclonal antibodies (MAb) is necessary to produce next generation MAbs and their derivatives such as and bispecific antibodies (bsAb) and single-chain variable fragments (scFv). OBJECTIVE: This study was aimed at cloning and characterization of the VH and VL cDNAs from a hybridoma cell against the CD20 antigen. METHODS: VH and VL fragments were amplified, cloned and characterized. Furthermore, amino acid sequences of VH, VL and corresponding complementarity-determining regions (CDR) were compared with those of four approved MAbs including Rituximab (RTX), Ibritumomab tiuxetan, Ofatumumab and GA101. RESULTS: The cloned VH and VL cDNAs were found to be functional and follow a consensus pattern. A noticeable similarity between the VH and VL amino acid sequences with those of RTX and Ibritumomab was evident. Furthermore, amino acid sequences of VH and VL CDRs indicated significant similarities to RTX and Ibritumomab CDRs. CONCLUSIONS: Successful recovery of VH and VL fragments encourages the development of novel CD20 targeting bsAbs, scFvs, antibody conjugates and T-cells armed with chimeric antigen receptors. |
| نویسنده | نفر چندم مقاله |
|---|---|
| داریوش شانه بندی | اول |
| جعفر مجیدی ذوالبین | دوم |
| توحید کاظمی | سوم |
| بهزاد برادران | چهارم |
| لیلی عاقبتی | پنجم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| shanehbandi2017.pdf | 1396/05/09 | 1296460 | دانلود |
