چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده ثبت کننده مقاله | جعفر مجیدی ذوالبین |
| مرحله جاری مقاله | تایید نهایی |
| دانشکده/مرکز مربوطه | مرکز تحقیقات ایمونولوژی |
| کد مقاله | 61090 |
| عنوان فارسی مقاله | Immuno-biosensor for Detection of CD20-Positive Cells Using Surface Plasmon Resonance |
| عنوان لاتین مقاله | Immuno-biosensor for Detection of CD20-Positive Cells Using Surface Plasmon Resonance |
| ناشر | 7 |
| آیا مقاله از طرح تحقیقاتی و یا منتورشیپ استخراج شده است؟ | بلی |
| عنوان نشریه (خارج از لیست فوق) | |
| نوع مقاله | Original Article |
| نحوه ایندکس شدن مقاله | ایندکس شده سطح یک – ISI - Web of Science |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | http://journals.tbzmed.ac.ir/APB/Manuscript/APB-7-189.pdf |
| Purpose: Surface plasmon resonance (SPR) sensing confers a real-time assessment of molecular interactions between biomolecules and their ligands. This approach is highly sensitive and reproducible and could be employed to confirm the successful binding of drugs to cell surface targets. The specific affinity of monoclonal antibodies (MAb) for their target antigens is being utilized for development of immuno-sensors and therapeutic agents. CD20 is a surface protein of B lymphocytes which has been widely employed for immunotargeting of B-cell related disorders. In the present study, binding ability of an anti-CD20 MAb to surface antigens of intact target cells was investigated by SPR technique. Methods: Two distinct strategies were used for immobilization of the anti-CD20 MAb onto gold (Au) chips. MUA (11-mercaptoundecanoic acid) and Staphylococcus aureus protein A (SpA) were the two systems used for this purpose. A suspension of CD20-positive Raji cells was injected in the analyte phase and the resulting interactions were analyzed and compared to those of MOLT-4 cell line as CD20-negative control. Results: Efficient binding of anti-CD20 MAb to the surface antigens of Raji cell line was confirmed by both immobilizing methods, whereas this MAb had not a noticeable affinity to the MOLT-4 cells. Conclusion: According to the outcomes, the investigated MAb had acceptable affinity and specificity to the target antigens on the cell surface and could be utilized for immunodetection of CD20-positive intact cells by SPR method. |
| نویسنده | نفر چندم مقاله |
|---|---|
| داریوش شانه بندی | اول |
| جعفر مجیدی ذوالبین | دوم |
| توحید کاظمی | سوم |
| بهزاد برادران | چهارم |
| لیلی عاقبتی | پنجم |
| فرزانه فتحی | ششم |
| جعفر عزتی نژاد دولت آبادی | هفتم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Immuno-biosensor for Detection of CD20-Positive Cells Using Surface.pdf | 1396/05/08 | 817374 | دانلود |
