چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده ثبت کننده مقاله | راحله فرحزادی |
| مرحله جاری مقاله | تایید نهایی |
| دانشکده/مرکز مربوطه | مرکز تحقیقات قلب وعروق |
| کد مقاله | 60855 |
| عنوان فارسی مقاله | Rat adipose-derived mesenchymal stem cells aging reduction by zinc sulfate under extremely low frequency electromagnetic field exposure is associated with increased telomerase reverse transcriptase gene expression |
| عنوان لاتین مقاله | Rat adipose-derived mesenchymal stem cells aging reduction by zinc sulfate under extremely low frequency electromagnetic field exposure is associated with increased telomerase reverse transcriptase gene expression |
| ناشر | 5 |
| آیا مقاله از طرح تحقیقاتی و یا منتورشیپ استخراج شده است؟ | بلی |
| عنوان نشریه (خارج از لیست فوق) | |
| نوع مقاله | Original Article |
| نحوه ایندکس شدن مقاله | ایندکس شده سطح یک – ISI - Web of Science |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5524544/ |
| Zinc as an essential trace element was reported to be involved in regulation of the growth and aging of cells. In this study, rat adipose-derived mesenchymal stem cells were exposed to extremely low frequency electromagnetic field (ELF-EMF) of 50 Hz and 20 mT to evaluate whether exposure to ELF-EMF in the presence of zinc sulfate (ZnSO4) affects the telomerase reverse transcriptase (TERT) gene expression and aging in mesenchymal stem cells (MSCs). The cell plates were divided into four groups including group I (control without ZnSO4 and ELF-EMF exposure); group II (ELF-EMF-exposure without ZnSO4); group III (ZnSO4 treatment without ELF-EMF exposure) and group ІV (ELF-EMF exposure with ZnSO4). In the presence of different concentrations of ZnSO4, cells viability, TERT gene expression and percentage of senescent cells were evaluated using colorimetric assay, real-time PCR and senescence-associated β-galactosidase activity assay, respectively. In this experiment, cells were exposed to ELF-EMF for 30 min per day for 21 days in the presence and absence of ZnSO4. The results revealed that ELF-EMF leads to a decrease in the expression of TERT gene and increase in the percentage of senescent cells. However, the ZnSO4 could significantly increase the TERT gene expression and decrease the aging of ELF-EMF-exposed MSCs. It seems that ZnSO4 may be a beneficial agent to delay aging of ELF-EMF-exposed MSCs due to the induction of TERT gene expression. |
| نویسنده | نفر چندم مقاله |
|---|---|
| عزت اله فتحی | اول |
| راحله فرحزادی | دوم |
| رضا رهبرقاضی | سوم |
| حسین صمدی کفیل | چهارم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| vrf-8-089.pdf | 1396/05/21 | 1110253 | دانلود |
