چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده ثبت کننده مقاله | محمدباقر حسینی |
| مرحله جاری مقاله | تایید نهایی |
| دانشکده/مرکز مربوطه | مرکز تحقیقات سلامت کودکان |
| کد مقاله | 60619 |
| عنوان فارسی مقاله | Evaluation of the Freeze–Thawing Method in Reducing Viral Load of Cytomegalovirus in Breast Milk of Mothers of Preterm Infants |
| عنوان لاتین مقاله | Evaluation of the Freeze–Thawing Method in Reducing Viral Load of Cytomegalovirus in Breast Milk of Mothers of Preterm Infants |
| ناشر | 8 |
| آیا مقاله از طرح تحقیقاتی و یا منتورشیپ استخراج شده است؟ | بلی |
| عنوان نشریه (خارج از لیست فوق) | |
| نوع مقاله | Original Article |
| نحوه ایندکس شدن مقاله | ایندکس شده سطح یک – ISI - Web of Science |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | http://online.liebertpub.com/doi/abs/10.1089/bfm.2016.0107?url_ver=Z39.88-2003&rfr_id=ori%3Arid%3Acrossref.org&rfr_dat=cr_pub%3D |
| Background: The aim of this study was to evaluate the freeze–thawing method in reducing viral load of cytomegalovirus (CMV) in breast milk of mothers of preterm infants. Methods and Materials: In this study, 169 nursing mothers of preterm infants were evaluated serologically for CMV infection. We found 29 (17.15%) nursing mothers who had serologic evidence of CMV infections consistent with recent infection (N = 2, IgG + IgM +) or prior infection (N= 27 IgG + IgM-). Two to 6 weeks after delivery, breast milk of seropositive mothers (N= 29) was evaluated for CMV load by real-time polymerase chain reaction (PCR) before and after freezing at -20C for 72 hours. All preterm infants with milk results positive for DNA particles of CMV (N = 25) were followed up for signs and symptoms of CMV infection until 3 months of age, together with urine testing for CMV by real-time PCR. Results: Examination of breast milk by real-time PCR of the seropositive mothers showed 25 of them with CMV DNA particles in their breast milk. After freezing and thawing, we found virus DNA in 4 of 25 (16%) of the breast milk samples and there was no CMV DNA particles in 21 (84%) of them. In these samples, mean viral load before freezing was 76.04 – 34.08 copies/lL (20–135.00 copies/lL) and after freezing it was 6.75 – 4.34 copies/lL (0.00–13.00 copies/lL). Freezing showed a significant decrease in viral load of the samples ( p = 0.001). Conclusion: The study showed that the freeze–thawing method is an effective method in reducing the CMV load in breast milk samples. |
| نویسنده | نفر چندم مقاله |
|---|---|
| محمدباقر حسینی | اول |
| حیدر علی اسماعیلی گاوگانی | دوم |
| شهرام عبدلی اسکویی | سوم |
| مرتضی قوجازاده | چهارم |
| زیبا مکاری یامچی | پنجم |
| وحیده لایق | ششم |
| لیلا امامی | هفتم |
| سامان مکاری | هشتم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| CMV Breast milk article (2).pdf | 1396/02/16 | 97957 | دانلود |
| Web of Science [v.5.pdf | 1396/10/24 | 161694 | دانلود |
