چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده ثبت کننده مقاله | حامد حاجی پور |
| مرحله جاری مقاله | تایید نهایی |
| دانشکده/مرکز مربوطه | دانشکده علوم نوین پزشکی |
| کد مقاله | 60546 |
| عنوان فارسی مقاله | ا پیگالوکاتچین 3 گالات (EGCG) از طریق افزایش بیان Bax و کاهش بیان Bcl-2 در رده سلولی سرطان پروستات (PC3) باعث القا آپوپتوز می شود. |
| عنوان لاتین مقاله | Epigallocatechin-3-Gallate Induces Apoptosis through Upregulation of Bax and Down-regulation of Bcl-2 in Prostate Cancer Cell Line |
| ناشر | 5 |
| آیا مقاله از طرح تحقیقاتی و یا منتورشیپ استخراج شده است؟ | خیر |
| عنوان نشریه (خارج از لیست فوق) | International Journal of Medical Laboratory |
| نوع مقاله | Original Article |
| نحوه ایندکس شدن مقاله | ایندکس شده سطح چهار – SID/Iranmedex/Magiran |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | http://ijml.ssu.ac.ir/article-1-148-en.html |
| Background and Aims: Epigallocatechin-3-gallate (EGCG) is a polyphenolic compound from green tea, which its anticancer effects on many types of cancers have been confirmed, but the molecular mechanism by which EGCG induces apoptosis remains unknown. The aim of the present study was to investigate anti-proliferative properties and apoptotic signaling pathway of EGCG on PC3 human prostate cancer cells. Materials and Methods: Cytotoxic effect of EGCG on prostate cancer cell line (PC3) was evaluated by MTT assay. DAPI staining was carried out to determine the morphological appearance of cells. Finally, the expression of Bax and Bcl-2 (apoptosis-regulating genes) were evaluated by quantitative Real-time polymerase chain reaction (PCR). Results: Cytotoxicity evaluations demonstrated that EGCG prevented prostate cancer cells growth in a time and dose depended manner, but the effect of treatment duration is more significant than effect of concentration. Cell growth inhibition was found to be accompanied by nucleus condensation or chromatin fragmentations which are signs of apoptosis, as assessed by DAPI staining. Quantitative Real-time PCR results demonstrated that EGCG causes upregulation of Bax as a pro-apoptotic protein, and down-regulation of Bcl-2 as an anti-apoptotic protein, thus shifting the Bax/Bcl-2 ratio in favor of apoptosis. Conclusions: It is tempting to suggest that consumption of EGCG could be an effective strategy to inhibit prostate cancer. Our results demonstrated that increase in the ratio of Bax/Bcl-2, is the probable molecular mechanisms through which EGCG induces apoptosis in PC3 cells. |
| نویسنده | نفر چندم مقاله |
|---|---|
| حامد حاجی پور | اول |
| حامد همیشه کار | دوم |
| سیامک برقی | چهارم |
| اکبر حسنی | پنجم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| 3.pdf | 1396/02/05 | 608647 | دانلود |
