چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده ثبت کننده مقاله | لیلا روشنگر |
| مرحله جاری مقاله | تایید نهایی |
| دانشکده/مرکز مربوطه | دانشکده پزشکی |
| کد مقاله | 60338 |
| عنوان فارسی مقاله | Study of chondrogenic potential of stem cells in co-culture with chondrons |
| عنوان لاتین مقاله | Study of chondrogenic potential of stem cells in co-culture with chondrons |
| ناشر | 9 |
| آیا مقاله از طرح تحقیقاتی و یا منتورشیپ استخراج شده است؟ | بلی |
| عنوان نشریه (خارج از لیست فوق) | |
| نوع مقاله | Original Article |
| نحوه ایندکس شدن مقاله | ایندکس شده سطح یک – ISI - Web of Science |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | http://ijbms.mums.ac.ir/article_7132.html |
| Objective(s): Three-dimensional biomimetic scaffolds have widespread applications in biomedical tissue engineering due to similarity of their nanofibrous architecture to native extracellular matrix. Co-culture system has stimulatory effect on chondrogenesis of adult mesenchymal stem cells. This work presents a co-culture strategy using human articular chondrons and adiposederived stem cells (ASCs) from infrapatellar fat pad (IPFP) for cartilage tissue production. Materials and Methods: Isolated stem cells were characterized by flowcytometry. Electrospun and polycaprolactone (PCL) scaffolds (900 nm fiber diameter) was obtained from Bon Yakhteh (Tehran- Iran) and human infrapatellar fat pad-derived stem cells (IPFP-ASCs) were seeded on them. IPFP- ASCs on scaffolds were co-cultured with articular chondrons using transwell. After 21 day, chondrogenic differentiation of stem cell was evaluated by determining the genes expression of collagen2, aggrecan and Indian hedgehog using real- time RT-PCR. Results: Genes expression of collagen2, aggrecan by IPFP-ASCs did not alter significantly in comparison with control group. Howevers, expression of Indian hedgehog decreased significantly compared to control group (P˂ 0.05). Conclusion: These findings indicate that chondrons obtained from osteoarthritic articular cartilage did not stimulate chondrogenic differentiation of IPFP-ASCs in co-culture. |
| نویسنده | نفر چندم مقاله |
|---|---|
| لیلا روشنگر | چهارم |
| داریوش محمدنژاد | دوم |
| هاجر شفائی | سوم |
| ناصر صمدی | پنجم |
| امیر محمد نوالی | ششم |
| علیرضا صادقپور تیمور لوئی | هفتم |
| داریوش شانه بندی | هشتم |
| جعفر سلیمانی راد | نهم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| IJBMS_Volume 19_Issue 6_Pages 638-645.pdf | 1395/12/23 | 1154139 | دانلود |
