چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده ثبت کننده مقاله | بهزاد برادران |
| مرحله جاری مقاله | تایید نهایی |
| دانشکده/مرکز مربوطه | مرکز تحقیقات ایمونولوژی |
| کد مقاله | 60217 |
| عنوان فارسی مقاله | اثرات درمانی Bach1 بر روی رده سلول سرطان سینه انسان |
| عنوان لاتین مقاله | Therapeutic effects of bach1 siRNA on human breast adenocarcinoma cell line |
| ناشر | 5 |
| آیا مقاله از طرح تحقیقاتی و یا منتورشیپ استخراج شده است؟ | خیر |
| عنوان نشریه (خارج از لیست فوق) | |
| نوع مقاله | Original Article |
| نحوه ایندکس شدن مقاله | ایندکس شده سطح یک – ISI - Web of Science |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | http://www.sciencedirect.com/science/article/pii/S0753332216316341 |
| Background: Despite the great improvements in clinical and therapeutic techniques in recent years, many advanced breast cancer patients still died of the postoperative recurrence and metastasis of disease. Bach1 plays a role in the development of the invasive phenotypes of cancer, cell division and apoptosis in tumor cells. The aim of this study was to investigate the effect of specific bach1 siRNAs, on the proliferation, migration, invasive, induction of apoptosis, cell cycle arrest and alter EMT related miRNA of MDA-MB-468 cells (breast cancer). Methods: siRNA transfection was performed with transfection reagent. The expression levels of Bach1 mRNA and protein were measured by qRT-PCR and western blot analysis, respectively. The survival of cells was determined using MTT assay cells, apoptosis using Tunel assay, Cell migration using scratch assay and Cell cycle analysis by Propidium Iodide (PI) DNA staining method by using flow cytometry on the MDA-MB-468. The expression levels of MMP-9 and CXCR4 were measured by qRT-PCR. Results: Transfection with siRNA significantly suppressed the expression of bach1 gene in dose dependent manner after 48 h (p < 0.0001). Surprisingly, treatment with bach1 siRNA arrest cell cycle in S phases (p < 0.0001). Moreover siRNA transfection had effects on breast adenocarcinoma cells and inhibits the migration (p < 0.0001), proliferation (p < 0.0001), cell cycle arrest (p = 0.03) and induces apoptosis (p < 0.0001) and reduces the expression of miR-21 (P = 0.0014). Conclusion: Our results suggest that the bach1 can be considered as a potent adjuvant in breast cancer therapy. |
| نویسنده | نفر چندم مقاله |
|---|---|
| بهزاد برادران | پنجم |
| بهزاد منصوری | دوم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| 177-Therapeutic effects of bach1 siRNA on human breast adenocarcinoma.pdf | 1395/12/12 | 1736676 | دانلود |
