| Objectives and Methods: This study was conducted to investigate in vitro, methanolic extract and methanol/water fractions obtainedfromthe
arial parts of Artemisia spicigera C.Koch(Asteraceae family)andfor evaluation of their antiproliferative effects against
HT-29, L-929 and A 549 cell lines by MTT assay at different concentrations (1, 10, 100, 1000 g/mL). Furthermore, this study aimed to
detect antimicrobial activities of the mentioned samples: two Gram-positive (Staphylococcus epidermidis and Staphylococcus aureus),
two Gram-negative bacteria (Pseudomonas aeruginosa and Escherichia coli) and a fungi (Candida albicanse), using agar well diffusion
method.
Results: The IC50 values for antiproliferative activity of the methanolic extract, 20%, 40% and 60% SPE (solid phase extraction) fractions,
found to be 345.9128.77, 442.4483.22, 220.1943.13 and 579.90153.19 g/mL, respectively; in this case, the maximum
inhibition percentage belonged to 40% SPE fraction (220.1943.13 g/mL). The total methanolic extract of A. spicigera indicated inhibitory
activity against Gram- positive strains, S. epidermidis and S. aureus, with MIC values of 150 mg/mL for both bacteria. Among
SPE fractions, 20% was the only active one against Gram possitive species with MIC values of 35 mg/mL for both strains.
Conclusions: This study revealed that methanolic extract of A. spicigera and its SPE fractions might be regarded as bioactive natural
products, which deserves to be further identification and isolation of cytotoxic and antibacterial phytochemicals from them. |