چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده ثبت کننده مقاله | لیلا روشنگر |
| مرحله جاری مقاله | تایید نهایی |
| دانشکده/مرکز مربوطه | دانشکده پزشکی |
| کد مقاله | 59594 |
| عنوان فارسی مقاله | Indirect coculture of stem cells with fetal chondrons using PCL electrospun nanofiber scaffolds |
| عنوان لاتین مقاله | Indirect coculture of stem cells with fetal chondrons using PCL electrospun nanofiber scaffolds |
| ناشر | 10 |
| آیا مقاله از طرح تحقیقاتی و یا منتورشیپ استخراج شده است؟ | بلی |
| عنوان نشریه (خارج از لیست فوق) | Artificial cells, nanomedicine, and biotechnology. |
| نوع مقاله | Original Article |
| نحوه ایندکس شدن مقاله | ایندکس شده سطح یک – ISI - Web of Science |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | http://www.tandfonline.com/loi/ianb20 |
| In vitro coculture system provides a powerful tool for tissue engineering. In this study, we evaluated the gene expressions of human adipose-derived stem cells (ASCs) on polycaprolactone (PCL) scaffold in coculture model with fetal chondrons. Electrospun PCL scaffolds (900nm fiber diameter) were created and human infrapatellar fat pad-adipose-derived stem cells (IPFP-ASCs) were seeded on these scaffolds. Scanning electron microscopy (SEM) showed attachment of human IPFP–ASCs to scaffold. IPFP-ASCs on scaffolds were cocultured with fetal chondrons in transwell. Gene expressions were investigated using real-time polymerase chain reaction (real-time PCR). In comparison with control group, the expression level of collagen type 2 and aggrecan were significantly decreased but Indian Hedgehog(IHH) significantly increased (P50.05).These findings may interpreted that IPFP-ASCs seeded on PCL scaffold, in cocultures with fetal chondrons are tending toward osteogenesis rather than chondrogenesis. |
| نویسنده | نفر چندم مقاله |
|---|---|
| جعفر سلیمانی راد | دوم |
| داریوش محمدنژاد | سوم |
| ناصر صمدی | چهارم |
| امیر محمد نوالی | ششم |
| هاجر شفائی | هفتم |
| حجت اله نوزاد چروده | هشتم |
| لیلا روشنگر | پنجم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Indirect coculture of stem cells with fetal chondrons using PCL electrospun nanofiber scaffolds.pdf | 1395/09/28 | 1252871 | دانلود |
