چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده ثبت کننده مقاله | ناصر صمدی |
| مرحله جاری مقاله | تایید نهایی |
| دانشکده/مرکز مربوطه | دانشکده علوم نوین پزشکی |
| کد مقاله | 59583 |
| عنوان فارسی مقاله | Multifunctional superparamagnetic nanoparticles: from synthesis to siRNA delivery. |
| عنوان لاتین مقاله | Multifunctional superparamagnetic nanoparticles: from synthesis to siRNA delivery. |
| ناشر | 6 |
| آیا مقاله از طرح تحقیقاتی و یا منتورشیپ استخراج شده است؟ | بلی |
| عنوان نشریه (خارج از لیست فوق) | |
| نوع مقاله | Original Article |
| نحوه ایندکس شدن مقاله | ایندکس شده سطح یک – ISI - Web of Science |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | https://benthamscience.com/journals/current-pharmaceutical-design/article/146864/ |
| Targeted delivery of small interfering RNA (siRNA) to specific tumor tissues and cells remains as one of the key challenges in the development of RNA interference as a therapeutic application. To target breast cancer, we developed a cationic nanoparticle as a therapeutic delivery system. The successful synthesis of the magnetic nanoparticles modified by polyaspartate (PAA) and polyethyleneimine (PEI) was confirmed using fourier transform infrared (FT-IR) measurements. The designed nanoparticle has been characterized evaluating its size and charge before and after nanoplex formation with siRNA. The designed nanoparticle could effectively form nanoplex with siRNA in 2:1 w/w ratio. Survivin siRNA was used to suppress the antiapoptotic gene, survivin, in MCF-7 cells. According to the importance of combinational therapy, Mitoxantrone (MTX) was used as a chemotherapeutic agent beside. The multifunctional nanoparticles have been successfully entered into about 63% of the MCF-7 cells which was shown via microscopic and flowcytometric methods. Effective cellular uptake led the cells to apoptosis. Down regulation of survivin was determined in mRNA and protein levels via Real Time PCR and western blotting. Gathering all obtained data, we came to conclusion that the Fe3O4-PAA-PEI nanoparticles can deliver siRNA effectively into the cytoplasm of the MCF-7 breast cancer cells and induce apoptosis. |
| نویسنده | نفر چندم مقاله |
|---|---|
| محمدرضا رشیدی شاهگلی | سوم |
| محمد سعید حجازی | پنجم |
| ناصر صمدی | ششم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| arami.pdf | 1398/02/20 | 2930285 | دانلود |
