چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| Extended-Spectrum β-Lactamase (ESBL) producing bacteria that can colonize the gut are often multi-drug resistant. The aim of this study was to determine ESBL-producing Escherichia coli (E. coli) isolated from the feces of healthy people in a small town of Iran. A total of 150 fecal samples from healthy humans were investigated in the community. These isolates were screened for ESBL production by the double disk synergy test. Susceptibility of isolated β-lactamase producing E. coli to 12 antimicrobial agents was tested by the disk diffusion method. The PCR was applied to detect CTX-M, SHV and TEM genes in ESBL-positive strains. Fifty-two out of 150 (34.6%) isolates were ESBL-producers. The majority of ESBL-producing isolates were resistant to co-trimoxazole, tetracycline and nalidixic acid. The CTX-M, SHV, and TEM genotypes were found in 86.5%, 9.6%, and 3.8% of ESBL-positive E. coli, respectively. The CTX-M1 is the most prevalent (80.7%) β-lactamase gene, followed by the SHV-12 and CTX-M14, TEM-116, CTX-M15, SHV-1 and CTX-M9. More than one-third of the E. coli isolates were ESBL producers. The high frequency of the CTX-M1 gene among E. coli isolates from the feces of healthy people is alarming. So, it is important to monitor ESBL-producing E. coli in order to avoid spread of resistant isolates in the community. |
| نویسنده | نفر چندم مقاله |
|---|---|
| رضا قوطاسلو | چهارم |
| محمد تقی اخی | دوم |
| لیلا یوسفی حلوایی | سوم |
| شیوا طاهری | اول |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| INTERNATIONAL JOURNAL OF ADVANCED BIOTECHNOLOGY AND RESEARCH, 7,4 2016r.pdf | 1397/01/18 | 257734 | دانلود |
| Doc1.docx | 1397/01/18 | 250533 | دانلود |
| Doc2.docx | 1397/01/18 | 400744 | دانلود |
| Doc3.docx | 1397/01/18 | 437162 | دانلود |
| Doc4.docx | 1397/01/18 | 213790 | دانلود |
