چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده ثبت کننده مقاله | علیرضا نورآذریان |
| مرحله جاری مقاله | تایید نهایی |
| دانشکده/مرکز مربوطه | دانشکده پزشکی |
| کد مقاله | 59480 |
| عنوان فارسی مقاله | اثر مرفین بروی قابلیت زیست پذیری ،نروآنژیوژنزو نرواسترئیدوژنز سلول های بنیادی عصبی رت |
| عنوان لاتین مقاله | The role of morphine on rat neural stem cells viability, neuro-angiogenesis and neuro-steroidgenesis properties |
| ناشر | 9 |
| آیا مقاله از طرح تحقیقاتی و یا منتورشیپ استخراج شده است؟ | بلی |
| عنوان نشریه (خارج از لیست فوق) | |
| نوع مقاله | Original Article |
| نحوه ایندکس شدن مقاله | ایندکس شده سطح یک – ISI - Web of Science |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | http://www.sciencedirect.com/science/article/pii/S0304394016308679 |
| A lack of comprehensive data exists on the effect of morphine on neural stem cell neuro-steroidogenesis and neuro-angiogenesis properties. We, herein, investigated the effects of morphine (100 M), naloxone (100 M) and their combination on rat neural stem cells viability, clonogenicity and Ki-67 expression over a period of 72 h. Any alterations in the total fatty acids profile under treatment protocols were elucidated by direct transesterification method. We also monitored the expression of p53, aromatase and 5-alpha reductase by real-time PCR assay. To examine angiogenic capacity, in vitro tubulogenesis and the level of VE-cadherin transcript were investigated during neural to endothelial differentiation under the experimental procedure. Cells supplemented with morphine displayed reduced survival (p < 0.01) and clonogenicity (p < 0.001). Flow cytometric analysis showed a decrease in Ki-67 during 72 h. Naloxone potentially blunted morphineinduced all effects. The normal levels of fatty acids, including saturated and unsaturated were altered by naloxone and morphine supplements. Following 48 h, the up-regulation of p53, aromatase and 5-alpha reductase genes occurred in morphine-primed cells. Using three-dimensional culture models of angiogenesis and real time PCR assay, we showed morphine impaired the tubulogenesis properties of neural stem cells (p < 0.001) by the inhibition of trans-differentiation into vascular cells and led to decrease of in VE-cadherin expression. Collectively, morphine strongly impaired the healthy status of neural stem cells by inducing p53 and concurrent elevation of aromatase and 5-alpha reductase activities especially during early 48 h. Also, neural stem cells-being exposed to morphine lost their potency to elicit angiogenesis. |
| نویسنده | نفر چندم مقاله |
|---|---|
| علیرضا نورآذریان | دوم |
| حجت اله نوزاد چروده | سوم |
| معصومه کاظمی | چهارم |
| رضا رهبرقاضی | نهم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| yazdani 2017.pdf | 1395/09/15 | 2618673 | دانلود |
