Molecular and Morphometric Characterization of Acanthamoeba spp. from Different Water Sources of Northwest Iran as a Neglected Focus, Co-Bordered With the Country of Iraq

Molecular and Morphometric Characterization of Acanthamoeba spp. from Different Water Sources of Northwest Iran as a Neglected Focus, Co-Bordered With the Country of Iraq


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نویسندگان: اسماعیل فلاح , مصطفی مصطفی زاده , عادل اسپوتین , آرام خضری , عباس شهبازی سیاه اسطلخی , محمود محامی اسکوئی , تیمور حضرتیان

کلمات کلیدی: Keywords: West Azerbaijan; Water resources; PCR; Iran; Acanthamoeba

نشریه: 22261 , 9 , 11 , 2016

اطلاعات کلی مقاله
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نویسنده ثبت کننده مقاله آرام خضری
مرحله جاری مقاله تایید نهایی
دانشکده/مرکز مربوطه دانشکده پزشکی
کد مقاله 59380
عنوان فارسی مقاله Molecular and Morphometric Characterization of Acanthamoeba spp. from Different Water Sources of Northwest Iran as a Neglected Focus, Co-Bordered With the Country of Iraq
عنوان لاتین مقاله Molecular and Morphometric Characterization of Acanthamoeba spp. from Different Water Sources of Northwest Iran as a Neglected Focus, Co-Bordered With the Country of Iraq
ناشر 7
آیا مقاله از طرح تحقیقاتی و یا منتورشیپ استخراج شده است؟ بلی
عنوان نشریه (خارج از لیست فوق)
نوع مقاله Original Article
نحوه ایندکس شدن مقاله ایندکس شده سطح یک – ISI - Web of Science
آدرس لینک مقاله/ همایش در شبکه اینترنت http://jjmicrobiol.com/?page=article&article_id=38481

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Background: Acanthamoeba spp. is a free-living opportunistic protozoan parasites, which can be found in tap, fresh and bottled mineral waters, contact lens solutions, soil etc.. Objectives: The present study is aimed to determine the Acanthamoeba spp. on the basis of their morpho-molecular aspects in different water sources of the West Azerbaijan province, Northwest of Iran.. Methods: In this cross-sectional study, 60 water samples were collected from rivers and tap waters during June to September 2015. The water samples were filtered through a cellulose nitrate filter and cultured on non-nutrient agar medium. The extracted DNAs were amplified and some ampliqons were sequenced using partial 18S rRNA for genotyping and phylogenetic analyses.. Results: Twenty-seven (45%) out of 60 water samples were positive to Acanthamoeba spp. using both culture and morphological examinations. In addition, 24 (40%) out of 27 positive samples in culture method were confirmed by PCR to be Acanthamoeba spp.. Conclusions: A relatively high prevalence of Acanthamoeba spp. in rivers reflects a risk alert for threatening human health in the region. However, well hygienic status of the tap waters considering Acanthamoeba spp. cannot be ignored in western co-border regions of Iran-Iraq. This study can also serve as a platform for further explorations of water sources in Iran and neighboring countries..

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نویسنده نفر چندم مقاله
اسماعیل فلاحدوم
مصطفی مصطفی زادهسوم
عادل اسپوتینچهارم
آرام خضریاول
عباس شهبازی سیاه اسطلخیپنجم
محمود محامی اسکوئیششم
تیمور حضرتیانهفتم

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