Molecular and Morphometric Characterization of Acanthamoeba spp. from Different Water Sources of Northwest Iran as a Neglected Focus, Co-Bordered With the Country of Iraq
Molecular and Morphometric Characterization of Acanthamoeba spp. from Different Water Sources of Northwest Iran as a Neglected Focus, Co-Bordered With the Country of Iraq
نویسندگان: اسماعیل فلاح , مصطفی مصطفی زاده , عادل اسپوتین , آرام خضری , عباس شهبازی سیاه اسطلخی , محمود محامی اسکوئی , تیمور حضرتیان
کلمات کلیدی: Keywords: West Azerbaijan; Water resources; PCR; Iran; Acanthamoeba
نشریه: 22261 , 9 , 11 , 2016
| نویسنده ثبت کننده مقاله |
آرام خضری |
| مرحله جاری مقاله |
تایید نهایی |
| دانشکده/مرکز مربوطه |
دانشکده پزشکی |
| کد مقاله |
59380 |
| عنوان فارسی مقاله |
Molecular and Morphometric Characterization of Acanthamoeba spp. from Different Water Sources of Northwest Iran as a Neglected Focus, Co-Bordered With the Country of Iraq |
| عنوان لاتین مقاله |
Molecular and Morphometric Characterization of Acanthamoeba spp. from Different Water Sources of Northwest Iran as a Neglected Focus, Co-Bordered With the Country of Iraq |
| ناشر |
7 |
| آیا مقاله از طرح تحقیقاتی و یا منتورشیپ استخراج شده است؟ |
بلی |
| عنوان نشریه (خارج از لیست فوق) |
|
| نوع مقاله |
Original Article |
| نحوه ایندکس شدن مقاله |
ایندکس شده سطح یک – ISI - Web of Science |
| آدرس لینک مقاله/ همایش در شبکه اینترنت |
http://jjmicrobiol.com/?page=article&article_id=38481 |
| Background: Acanthamoeba spp. is a free-living opportunistic protozoan parasites, which can be found in tap, fresh and bottled mineral waters, contact lens solutions, soil etc..
Objectives: The present study is aimed to determine the Acanthamoeba spp. on the basis of their morpho-molecular aspects in different water sources of the West Azerbaijan province, Northwest of Iran..
Methods: In this cross-sectional study, 60 water samples were collected from rivers and tap waters during June to September 2015. The water samples were filtered through a cellulose nitrate filter and cultured on non-nutrient agar medium. The extracted DNAs were amplified and some ampliqons were sequenced using partial 18S rRNA for genotyping and phylogenetic analyses..
Results: Twenty-seven (45%) out of 60 water samples were positive to Acanthamoeba spp. using both culture and morphological examinations. In addition, 24 (40%) out of 27 positive samples in culture method were confirmed by PCR to be Acanthamoeba spp..
Conclusions: A relatively high prevalence of Acanthamoeba spp. in rivers reflects a risk alert for threatening human health in the region. However, well hygienic status of the tap waters considering Acanthamoeba spp. cannot be ignored in western co-border regions of Iran-Iraq. This study can also serve as a platform for further explorations of water sources in Iran and neighboring countries.. |
| نام فایل |
تاریخ درج فایل |
اندازه فایل |
دانلود |
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