Advantages of Sheep Infrapatellar Fat Pad Adipose Tissue Derived Stem Cells in Tissue Engineering
Advantages of Sheep Infrapatellar Fat Pad Adipose Tissue Derived Stem Cells in Tissue Engineering
نویسندگان: پرویز واحدی , جعفر سلیمانی راد , لیلا روشنگر , هاجر شفائی , حجت اله نوزاد چروده , سیدحسین جارالمسجد
کلمات کلیدی: Anti-Human Antibodies, Infrapatellar fat pad adipose tissue, CD markers, ASCs
نشریه: 951 , 1 , 6 , 2016
| نویسنده ثبت کننده مقاله |
لیلا روشنگر |
| مرحله جاری مقاله |
تایید نهایی |
| دانشکده/مرکز مربوطه |
دانشکده پزشکی |
| کد مقاله |
59173 |
| عنوان فارسی مقاله |
Advantages of Sheep Infrapatellar Fat Pad Adipose Tissue Derived Stem Cells in Tissue Engineering |
| عنوان لاتین مقاله |
Advantages of Sheep Infrapatellar Fat Pad Adipose Tissue Derived Stem Cells in Tissue Engineering |
| ناشر |
6 |
| آیا مقاله از طرح تحقیقاتی و یا منتورشیپ استخراج شده است؟ |
بلی |
| عنوان نشریه (خارج از لیست فوق) |
|
| نوع مقاله |
Original Article |
| نحوه ایندکس شدن مقاله |
ایندکس شده سطح یک – ISI - Web of Science |
| آدرس لینک مقاله/ همایش در شبکه اینترنت |
- |
| Purpose: The goal of this study has been to evaluate adipose tissue derived stem cells
(ADSCs) from infrapatellar fat pad and characterize their cell surface markers using antihuman
antibodies, as adipose tissue derived stem cells (ADSCs) have great potential for
cellular therapies to restore injured tissues.
Methods: Adipose tissue was obtained from infrapatellar fat pad of sheep. Surface markers
evaluated by flow cytometry. In order to evaluate cell adhesion, the Polycaprolactone (PCL)
was sterilized under Ultraviolet (UV) light and about 1×105 cells were seeded on PCL.
Then, ASCs- PCL construct were evaluated by Scanning Electron Microscopy (Mira3 Te
Scan, Czech Republic).
Results: We showed that adipose tissue derived stem cells (ADSCs) maintain their
fibroblastic-like morphology during different subcultures and cell adhesion. They were
positive for CD44 and CD90 markers and negative for CD31 and Cd45 markers by human
antibodies.
Conclusion: Our results suggest that ASCs surface markers can be characterized by antihuman
antibodies in sheep. As stem cells, they can be used in tissue engineering. |
| نام فایل |
تاریخ درج فایل |
اندازه فایل |
دانلود |
| apb-6-105 vahedi.pdf | 1395/08/11 | 1334806 | دانلود |