چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده ثبت کننده مقاله | کریم شمس اسنجان |
| مرحله جاری مقاله | تایید نهایی |
| دانشکده/مرکز مربوطه | دانشکده پزشکی |
| کد مقاله | 58793 |
| عنوان فارسی مقاله | Galectin-1 supports the survival of CD45RA(-) primary myeloma cells in vitro |
| عنوان لاتین مقاله | Galectin-1 supports the survival of CD45RA(-) primary myeloma cells in vitro |
| ناشر | 9 |
| آیا مقاله از طرح تحقیقاتی و یا منتورشیپ استخراج شده است؟ | خیر |
| عنوان نشریه (خارج از لیست فوق) | |
| نوع مقاله | Original Article |
| نحوه ایندکس شدن مقاله | ایندکس شده سطح یک – ISI - Web of Science |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | http://www.ncbi.nlm.nih.gov/pubmed/18537967 |
| Galectin-1 supports the survival of CD45RA()) primary myeloma cells in vitro Summary The survival and proliferation of human myeloma cells are considered to be heavily dependent on the microenvironment of bone marrow (BM). This study confirmed that galectin-1 (Gal-1) and SDF-1a were produced by bone marrow mononuclear cells of myeloma patients. The addition of Gal-1 and SDF-1a to a serum-free synthetic medium, maintained the viability of primary myeloma cells for 2 weeks similar to that before culture. While Gal-1 reduced the viable cell number in CD45RA(+) B cell lines, it maintained the viability of CD45()) U266 and CD45RA())RO(+) ILKM3 myeloma cell lines in the synthetic medium. This was confirmed with the transfection of the PTPRC (CD45) RA, -RB, or -RO gene into CD45()) U266 cells. The combination of Gal-1 and SDF-1a significantly induced phosphorylation of Akt and IkB, while the phosphorylation of ERK1/2 was significantly reduced in CD45RA(+) U266 and Raji cells but not CD45()) or CD45RA()) U266 cells. Furthermore, we confirmed that Gal-1 bound to CD45RA in CD45RA(+) Raji cells, and also physically interacted with b1-integrin by immunoprecipitation followed by Western blotting and confocal microscopy. The results suggest that Gal-1 has two different actions depending on its binding partner, and supports the survival of CD45RA()) myeloma cells. |
| نویسنده | نفر چندم مقاله |
|---|---|
| کریم شمس اسنجان | سوم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Galectin-1 supports the survival of CD45RA(-) primary myeloma cells in vitro.pdf | 1395/06/29 | 476011 | دانلود |
