چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده ثبت کننده مقاله | کریم شمس اسنجان |
| مرحله جاری مقاله | تایید نهایی |
| دانشکده/مرکز مربوطه | مرکز تحقیقات هماتولوژی و انکولوژی |
| کد مقاله | 58768 |
| عنوان فارسی مقاله | Time - and concentration - dependent effects of resveratrol on miR 15a and miR16-1 expression and apoptosis CCRF-CEM acute lymphoblastic leukemia cell line |
| عنوان لاتین مقاله | Time - and concentration - dependent effects of resveratrol on miR 15a and miR16-1 expression and apoptosis CCRF-CEM acute lymphoblastic leukemia cell line |
| ناشر | 11 |
| آیا مقاله از طرح تحقیقاتی و یا منتورشیپ استخراج شده است؟ | بلی |
| عنوان نشریه (خارج از لیست فوق) | |
| نوع مقاله | Original Article |
| نحوه ایندکس شدن مقاله | ایندکس شده سطح دو – PubMed |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | http://www.ncbi.nlm.nih.gov/pubmed/26434860 |
| Time--and Concentration--Dependent Effects of Resveratrol on miR 15a and miR16-1 Expression and Apoptosis in the CCRF-CEM Acute Lymphoblastic Leukemia Cell Line. Abstract BACKGROUND: Chemotherapy is one of the common approaches in treatment of cancers, especially leukemia. However, drug resistance phenomena reduce the likelihood of treatment success. Resveratrol is a herbal compound which through complicated processes makes some selected cells sensitive to treatment and induction of apoptosis. In the present study, the effects of resveratrol on the expression of miR 15a and miR16-1 and apoptosis in the CCRF-CEM cell line were investigated. MATERIALS AND METHODS: The CCRF-CEM cell line was cultured under standard conditions and changes in miR 15a and miR 16-1 expression were analyzed by real time-PCR technique, with attention to reveratrol dose and time dependence. Also, apoptosis is evaluated by flow cytometry using annexin V and PI. RESULTS: CCRF-CEM cells underwent dose-dependent apoptotic cell death in response to resveratrol. MiR 15a and miR 16-1 expression was up-regulated after 24 and 48 hours resveratrol treatment (p<0.05). CONCLUSIONS: The results of our study indicate that resveratrol induces apoptosis in a time and dose- dependent manner in CCRF-CEM cells. Also, increased expression level of miR 16-1 and miR 15a by means of resveratrol in CCRF-CEM cells might have a role in apoptosis induction and predisposition. According to our results resveratrol can be regarded as a dietary supplement to improve efficacy of anti-leukemia therapies. |
| نویسنده | نفر چندم مقاله |
|---|---|
| کریم شمس اسنجان | هشتم |
| آکو عظیمی | اول |
| مجید فرش دوستی حق | دوم |
| مهدی طالبی | سوم |
| بهمن یوسفی | چهارم |
| عباسعلی حسین پورفیضی | پنجم |
| بهزاد برادران | ششم |
| علی اکبر موثق پور اکبری | هفتم |
| تقی خان زاده | نهم |
| میلاد زادی حیدرآباد | یازدهم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Time - and Concentration - Dependent Effects of Resveratrol.pdf | 1395/06/28 | 752291 | دانلود |
