فراوانی الل نول HLA-G انسان در افراد با سقط مکرر

Frequency of null allele of Human Leukocyte Antigen-G (HLA-G) locus in subjects to recurrent miscarriage


چاپ صفحه		 پژوهان
صفحه نخست سامانه		 چکیده مقاله
چکیده مقاله		 نویسندگان
نویسندگان		 دانلود مقاله
دانلود مقاله		 دانشگاه علوم پزشکی تبریز
دانشگاه علوم پزشکی تبریز

نویسندگان: بهزاد برادران

کلمات کلیدی: Allele, HLA-G*0105N, Recurrent miscarriage, RFLPs.

نشریه: 16606 , 7 , 14 , 2016

اطلاعات کلی مقاله
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نویسنده ثبت کننده مقاله بهزاد برادران
مرحله جاری مقاله تایید نهایی
دانشکده/مرکز مربوطه مرکز تحقیقات ایمونولوژی
کد مقاله 58740
عنوان فارسی مقاله فراوانی الل نول HLA-G انسان در افراد با سقط مکرر
عنوان لاتین مقاله Frequency of null allele of Human Leukocyte Antigen-G (HLA-G) locus in subjects to recurrent miscarriage
ناشر 7
آیا مقاله از طرح تحقیقاتی و یا منتورشیپ استخراج شده است؟ خیر
عنوان نشریه (خارج از لیست فوق) International Journal of Reproductive BioMedicine
نوع مقاله Original Article
نحوه ایندکس شدن مقاله ایندکس شده سطح یک – ISI - Web of Science
آدرس لینک مقاله/ همایش در شبکه اینترنت http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4971563/

خلاصه مقاله
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Background: Human leukocyte antigen-G (HLA-G) is a non-classical class I molecule highly expressed by extravillous cytotrophoblast cells. Due to a single base pair deletion, its function can be compensated by other isoforms. Investigating the frequency of null allele in Recurrent Miscarriage (RM) subjects could be useful in understanding the relationship between frequency of this allele and RM in a given population. Objective: This study aimed to determine the frequency of HLA-G*0105N null allele and its potential association with down-regulation of HLA-G in subjects with RM. Materials and Methods: Western blotting was used to assess the level of HLA-G protein expression. For investigating the frequency of HLA-G*0105N null allele in RM subjects, PCR-RFLP method was used. Exon 3 of HLA-G gene was amplified by polymerase chain reaction (PCR). Subsequently, PpuM-1 enzyme was employed to digest the PCR products and fragments were analyzed using gel electrophoresis. Results: Digestion using restriction enzyme showed the presence of heterozygous HLA-G*0105N null allele in 10% of the test population. Western blotting results confirmed the decrease in expression of HLA-G in the placental tissue of subjects with RM compared to subjects who could give normal birth. Conclusion: The frequency of heterozygous HLA-G*0105N null allele was high to some extent in subjects with RM. The mutation rate in subjects suggested that there is a significant association between RM and frequency of mutations in this allele.

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نویسنده نفر چندم مقاله
بهزاد برادرانهفتم

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167-Frequency of null allele of Human Leukocyte.pdf1395/06/23236114دانلود