چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده ثبت کننده مقاله | بهمن اکبری |
| مرحله جاری مقاله | تایید نهایی |
| دانشکده/مرکز مربوطه | دانشکده علوم نوین پزشکی |
| کد مقاله | 58395 |
| عنوان فارسی مقاله | ساخت و ارزیابی یک آنتی بادی تک زنجیره ای انسانی شده علیه تومورهای با بیان بالای EGFR |
| عنوان لاتین مقاله | Construction and evaluation of a humanized single-chain antibody (huscFv)against EGFR-overexpressing tumors |
| نوع ارائه | سخنرانی |
| عنوان کنگره / همایش | 13th International Congress of Immunology & Allergy of Iran |
| نوع کنگره / همایش | سخنرانی |
| کشور محل برگزاری کنگره/ همایش | ایران - تبریز |
| شهر محل برگزاری کنگره/ همایش | تبریز |
| سال انتشار/ ارائه شمسی | 1395 |
| سال انتشار/ارائه میلادی | 2016 |
| تاریخ شمسی شروع و خاتمه کنگره/همایش | 1395/02/07 الی 1395/02/10 |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | http://icia.ir/ |
| آدرس علمی (Affiliation) نویسنده متقاضی | Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, East Azerbaijan, Iran |
| نویسنده | نفر چندم مقاله |
|---|---|
| صفر فرج نیا | دوم |
| بهمن اکبری | اول |
| نصرت اله ضرغامی | سوم |
| شیوا عهدی خسروشاهی | پنجم |
| عنوان | متن |
|---|---|
| خلاصه مقاله | Introduction: Targeted cancer therapeutics are drugs that block the growth of cancer by interfering with specific molecules of cancer cells. Monoclonal antibodies are among the most specific agents recognized and bind to cancer cell surface antigens. Several recombinant formats of monoclonal antibodies have been developed such as full length antibody, Fab, scFv and etc.ScFvs are powerful tools and new reagents in biological research, diagnostic imaging and tumor therapy. Humanized scFv antibodies have been proposed to reduce the size and immunogenicity of murine and chimeric full length antibody. Here we describe the production of an anti-EGFR huscFv employing CDR-grafting method. Materials and methods: In the design of desired huscFv, the murine scFv sequence was humanized by CDR-grafting method. The huscFv was cloned and expressed in E. coli and affinity purified. The reactivity of huscFv was assessed by ELISA and the MTT assay was employed to assess the cell growth inhibition of huscFv on A431 cells. Result: The humanization of murine variable regions resulted in 13.7% increase inhumanness of hscFv. Expression analysis showed that the desired huscFv was produced with high concentration in E. coli. Investigation of hscFv reactivity on A431 cells revealed higher binding affinity and better growth inhibition capability comparing to murine counterpart. Conclusion: Results of reactivity and inhibition assay indicates that hscFv produced in this study can serve as a potent humanized single chain antibody to target EGFR- overexpressing tumor cells. |
| کلمات کلیدی | Key word: Cancer targetedtherapy, huscFv, CDR-grafting |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Abstract book first page.jpg | 1395/05/02 | 75230 | دانلود |
| karbarge.docx | 1395/05/18 | 15803 | دانلود |
| moshakhasate daneshjo.docx | 1395/05/18 | 13381 | دانلود |
| HuscFv .pdf | 1395/05/18 | 12899 | دانلود |
| Certificate.png | 1395/05/18 | 12376033 | دانلود |
